The EMBO Journal

Developmental regulation of CYCA2s contributes to tissue-specific proliferation in Arabidopsis

In multicellular organisms, morphogenesis relies on a strict coordination in time and space of cell proliferation and differentiation. In contrast to animals, plant development displays continuous organ formation and adaptive growth responses during their lifespan relying on a tight coordination of cell proliferation. How developmental signals interact with the plant cell-cycle machinery is largely unknown. Here, we characterize plant A2-type cyclins, a small gene family of mitotic cyclins, and show how they contribute to the fine-tuning of local proliferation during plant development. Moreover, the timely repression of CYCA2;3 expression in newly formed guard cells is shown to require the stomatal transcription factors FOUR LIPS/MYB124 and MYB88, providing a direct link between developmental programming and cell-cycle exit in plants. Thus, transcriptional downregulation of CYCA2s represents a critical mechanism to coordinate proliferation during plant development.

Steffen Vanneste1,2,7, Frederik Coppens1,2,7, EunKyoung Lee3, Tyler J Donner4, Zidian Xie5, Gert Van Isterdael1,2, Stijn Dhondt1,2, Freya De Winter1,2, Bert De Rybel1,2,8, Marnik Vuylsteke1,2, Lieven De Veylder1,2, Jiří Friml1,2, Dirk Inzé1,2, Erich Grotewold5, Enrico Scarpella4, Fred Sack3, Gerrit T S Beemster1,2,6 and Tom Beeckman1,2

  1. Department of Plant Systems Biology, VIB, Ghent, Belgium
  2. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium
  3. Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada
  4. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
  5. Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center, The Ohio State University, Columbus, OH, USA
  6. Department of Biology, University of Antwerp, Antwerp, Belgium

The EMBO Journal (2011) 30, 3430 – 3441. Full paper here.