2D Plots of Microarray Data
See Also  Example
This menu can be used to produce 2D plots of microarray data or transformed data using log base 2. You can display separate plots for all slides or use a subset of these. Alternatively, you can display all the plots within a single graph using a trellis plot. For each of the plots you can add a reference and mean curve.

Available Data

This lists data structures appropriate for the edit box which currently has focus. You can double-click a name to enter it in the edit box.

Y Variate

The variate(s) to be plotted on the Y axis of the plot.

X Variate

The variate(s) to be plotted on the X axis of the plot.

Use Log (base 2) Transformation

The Y and X variates are log 2 transformed before plotting. The calculation for this using GenStat's command language is:

CALC LogY = LOG(Y)/LOG(2)

Slides

The factor that identifies the slides. If just a single slide is being displayed, this does not needed to be provided.

Subset of Slides to Plot

If you only want plots of some of the slides in the data set, you can provide a list of the levels or labels of the slides you want. The labels should be separated by commas. If the labels have spaces in them, quote them with single quotes, e.g. 'MS 1','MS 2', 'MS 3'.

Groups within Plots

A factor that indicates groups within each graph which are to be plotted in different colours.

Action Buttons

RunGenerate the graphs.
CancelClose the menu without further changes.
OptionsOpens a dialog where additional options and settings can be specified for the graphs.
DefaultsSet the menu settings back to the default settings. Clicking the right mouse on this button produces a shortcut menu where you can choose to set the menu using the currently stored defaults or the GenStat default settings.

Example

The following example shows how to plot the foreground means of the red and green dyes against each other (i.e. F1Mean vs F2Mean), with different print tips (in factor Slide_block) for a subset of 9 slides in the experiment.

The options have the titles provided, and specify a 1-1 reference line within a trellis plot.

This produces in the following graph:

See Also